Record Details

Title:
Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
Affiliation(s):
EuXFEL staff, Other
Author group:
Advanced Electronics
Topic:
Abstract:
Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.
Imprint:
2016
Journal Information:
Scientific Data, 3, 160060 (2016)
Related external records:
Language(s):
English


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 Record created 2016-12-05, last modified 2019-07-30

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