Record Details

Title:
Single mimivirus particles intercepted and imaged with an x-ray laser
Affiliation(s):
EuXFEL guest, EuXFEL staff
Author group:
Advanced Electronics
Management Board
Instrument SPB/SFX
Sample Environment
Detector Development
Keyword(s):
Abstract:
X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval. Here we show that high-quality diffraction data can be obtained with a single X-ray pulse from a non-crystalline biological sample, a single mimivirus particle, which was injected into the pulsed beam of a hard-X-ray free-electron laser, the Linac Coherent Light Source. Calculations indicate that the energy deposited into the virus by the pulse heated the particle to over 100,000 K after the pulse had left the sample. The reconstructed exit wavefront (image) yielded 32-nm full-period resolution in a single exposure and showed no measurable damage. The reconstruction indicates inhomogeneous arrangement of dense material inside the virion. We expect that significantly higher resolutions will be achieved in such experiments with shorter and brighter photon pulses focused to a smaller area. The resolution in such experiments can be further extended for samples available in multiple identical copies.
Imprint:
London [u.a.], Nature Publising Group, 2011
Journal Information:
Nature <London>, Vol. 470, p. 78-81, 470<78-81 (2011)
ISSN:
1476-4687
0028-0836
Related external records:
View record in PubMed
WOS: WOS:000286886400037
DOI: 10.1038/nature09748
Language(s):
English


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 Record created 2016-10-11, last modified 2019-02-08

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