Record Details

Title:
In vivo protein crystallization opens new routes in structural biology
Affiliation(s):
EuXFEL guest, EuXFEL staff
Author group:
Optical Lasers
Advanced Electronics
Instrument SPB/SFX
Sample Environment
Detector Development
Keyword(s):
Abstract:
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
Imprint:
London [u.a.] Nature Publishing Group, Nature Publishing Group, 2012
Journal Information:
Nat. Methods, 9, 259-262 (2012)
ISSN:
1548-7105
1548-7091
Related external records:
View record in PubMed
pmc: pmc:PMC3429599
WOS: WOS:000300890400020
DOI: 10.1038/nmeth.1859
Language(s):
English


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 Record created 2016-10-11, last modified 2019-02-08


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