Record Details

Title:
Hard X-ray imaging of bacterial cells: nano-diffraction and ptychographic reconstruction
Affiliation(s):
EuXFEL staff
Author group:
Instrument SPB/SFX
Keyword(s):
Topic:
Abstract:
Ptychographic coherent X-ray diffractive imaging (PCDI) has been combined with nano-focus X-ray diffraction to study the structure and density distribution of unstained and unsliced bacterial cells, using a hard X-ray beam of 6.2keV photon energy, focused to about 90nm by a Fresnel zone plate lens. While PCDI provides images of the bacteria with quantitative contrast in real space with a resolution well below the beam size at the sample, spatially resolved small angle X-ray scattering using the same Fresnel zone plate (cellular nano-diffraction) provides structural information at highest resolution in reciprocal space up to 2nm(-1). We show how the real and reciprocal space approach can be used synergistically on the same sample and with the same setup. In addition, we present 3D hard X-ray imaging of unstained bacterial cells by a combination of ptychography and tomography.
Imprint:
Washington, DC, Soc., 2012
Journal Information:
Opt. Express, 20, 19232-19254 (2012)
ISSN:
1094-4087
Related external records:
View record in PubMed
DOI: 10.1364/OE.20.019232
WOS: WOS:000307873600075
Language(s):
English


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 Record created 2016-10-11, last modified 2019-02-08


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