Record Details

Title:
Single-particle structure determination by correlations of snapshot X-ray diffraction patterns
Affiliation(s):
EuXFEL staff
Author group:
Detector Development
Sample Environment
Management Board
Advanced Electronics
Abstract:
Diffractive imaging with free-electron lasers allows structure determination from ensembles of weakly scattering identical nanoparticles. The ultra-short, ultra-bright X-ray pulses provide snapshots of the randomly oriented particles frozen in time, and terminate before the onset of structural damage. As signal strength diminishes for small particles, the synthesis of a three-dimensional diffraction volume requires simultaneous involvement of all data. Here we report the first application of a three-dimensional spatial frequency correlation analysis to carry out this synthesis from noisy single-particle femtosecond X-ray diffraction patterns of nearly identical samples in random and unknown orientations, collected at the Linac Coherent Light Source. Our demonstration uses unsupported test particles created via aerosol self-assembly, and composed of two polystyrene spheres of equal diameter. The correlation analysis avoids the need for orientation determination entirely. This method may be applied to the structural determination of biological macromolecules in solution.
Imprint:
London, Nature Publishing Group, 2012
Journal Information:
Nat. Commun., 3, 1276 (2012)
ISSN:
2041-1723
Related external records:
View record in PubMed
DOI: 10.1038/ncomms2288
WOS: WOS:000316356700043
Language(s):
English


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 Record created 2016-10-11, last modified 2019-02-08

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