000003891 001__ 3891
000003891 005__ 20241118073825.0
000003891 02470 $$2DOI$$a10.1016/bs.mie.2024.10.007
000003891 037__ $$aARTICLE
000003891 041__ $$aeng
000003891 245__ $$aSample delivery for structural biology at the European XFEL
000003891 260__ $$bElsevier B.V.$$c2024
000003891 269__ $$a2024
000003891 336__ $$aArticles
000003891 520__ $$aSerial femtosecond crystallography (SFX) at X-ray free electron lasers (XFELs) is a valuable technique for time-resolved structural studies on enzymes. This method allows for the collection of high-resolution datasets of protein structures at various time points during a reaction initiated by light or mixing. Experiments are performed under non-cryogenic conditions and allow the collection of radiation damage free structures. At the European XFEL (EuXFEL), SFX experiments are mainly performed with liquid jets produced by gas dynamic virtual nozzles (GDVNs) and less frequent with a high viscous extruder (HVE). In this chapter we describe these delivery methods, with the focus on GDVNs. Instrumentation, sample requirements, and preparation steps for SFX beamtimes are discussed. Other sample delivery methods available at the EuXFEL are briefly introduced at the end of this chapter.
000003891 546__ $$aEnglish
000003891 655__ $$aX-ray scattering, X-ray optics, and instrumentation techniques
000003891 655__ $$aStructural biology and biocrystallography
000003891 690__ $$aEuXFEL facility
000003891 690__ $$aExperimental techniques
000003891 7001_ $$aDörner, Katerina$$0XFEL915
000003891 7001_ $$aSmyth, Peter$$0XFEL1707
000003891 7001_ $$aSchulz, Joachim$$0XFEL289
000003891 773__ $$j1$$pMethods Enzymol$$qearly access$$tMethods in Enzymology
000003891 790__ $$aEuXFEL staff
000003891 85641 $$uhttps://www.sciencedirect.com/science/article/abs/pii/S0076687924005251?via%3Dihub
000003891 8564_ $$uhttps://xfel.tind.io/record/3891/files/10.1016_bs.mie.2024.10.007.pdf$$922d6180c-c688-49b5-bf05-b033c258c2af$$s4427622
000003891 900__ $$aSample Environment
000003891 909CO $$ooai:xfel.tind.io:3891$$pGLOBAL_SET
000003891 955__ $$amerret.kleesattel@xfel.eu
000003891 980__ $$aARTICLE
000003891 981__ $$aoverwrite