Record Details

Title:
Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
Affiliation(s):
EuXFEL guest
Author group:
Instrument SPB/SFX
Topic:
Abstract:
We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions
Imprint:
London [u.a.] Nature Publishing Group, Nature Publishing Group, 2014
Journal Information:
Nat. Methods, 11, 9, 923-926 (2014)
ISSN:
1548-7091
1548-7105
Related external records:
DOI: 10.1038/nmeth.3067
WOS: WOS:000341409700019


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 Record created 2016-10-11, last modified 2019-02-08

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