Record Details

Title:
X-ray photon correlation spectroscopy of protein dynamics at nearly diffraction-limited storage rings
Affiliation(s):
EuXFEL staff, Other
Author group:
Instrument MID
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Abstract:
This study explores the possibility of measuring the dynamics of proteins in solution using X-ray photon correlation spectroscopy (XPCS) at nearly diffraction-limited storage rings (DLSRs). We calculate the signal-to-noise ratio (SNR) of XPCS experiments from a concentrated lysozyme solution at the length scale of the hydrodynamic radius of the protein molecule. We take into account limitations given by the critical X-ray dose and find expressions for the SNR as a function of beam size, sample-to-detector distance and photon energy. Specifically, we show that the combined increase in coherent flux and coherence lengths at the DLSR PETRA IV yields an increase in SNR of more than one order of magnitude. The resulting SNR values indicate that XPCS experiments of biological macromolecules on nanometre length scales will become feasible with the advent of a new generation of synchrotron sources. Our findings provide valuable input for the design and construction of future XPCS beamlines at DLSRs.
Imprint:
International Union of Crystallography, 2019
Journal Information:
IUCrJ, 6 (5), 1-10 (2019)
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Language(s):
English


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 Record created 2019-07-26, last modified 2019-07-26

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