000001940 001__ 1940
000001940 005__ 20190416130814.0
000001940 02470 $$2DOI$$a10.1107/S1600577519003308
000001940 037__ $$aARTICLE-2019-061
000001940 041__ $$aeng
000001940 245__ $$aThe Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European XFEL: initial installation
000001940 260__ $$c2019
000001940 269__ $$a2019
000001940 336__ $$aArticles
000001940 520__ $$aThe European X-ray Free-Electron Laser (FEL) became the first operational high-repetition-rate hard X-ray FEL with first lasing in May 2017. Biological structure determination has already benefitted from the unique properties and capabilities of X-ray FELs, predominantly through the development and application of serial crystallography. The possibility of now performing such experiments at data rates more than an order of magnitude greater than previous X-ray FELs enables not only a higher rate of discovery but also new classes of experiments previously not feasible at lower data rates. One example is time-resolved experiments requiring a higher number of time steps for interpretation, or structure determination from samples with low hit rates in conventional X-ray FEL serial crystallography. Following first lasing at the European XFEL, initial commissioning and operation occurred at two scientific instruments, one of which is the Single Particles, Clusters and Biomolecules and Serial Femtosecond Crystallography (SPB/SFX) instrument. This instrument provides a photon energy range, focal spot sizes and diagnostic tools necessary for structure determination of biological specimens. The instrumentation explicitly addresses serial crystallography and the developing single particle imaging method as well as other forward-scattering and diffraction techniques. This paper describes the major science cases of SPB/SFX and its initial instrumentation – in particular its optical systems, available sample delivery methods, 2D detectors, supporting optical laser systems and key diagnostic components. The present capabilities of the instrument will be reviewed and a brief outlook of its future capabilities is also described.
000001940 546__ $$aEnglish
000001940 6531_ $$aXFEL
000001940 6531_ $$aserial crystallography
000001940 6531_ $$ainstrumentation
000001940 655__ $$aStructural biology and biocrystallography
000001940 655__ $$aX-ray scattering, X-ray optics, and instrumentation techniques
000001940 690__ $$aEuXFEL facility
000001940 7001_ $$aMancuso, Adrian P.
000001940 7001_ $$aAquila, Andrew
000001940 7001_ $$aBatchelor, Lewis
000001940 7001_ $$aBean, Richard J.
000001940 7001_ $$aBielecki, Johan
000001940 7001_ $$aBorchers, Gannon
000001940 7001_ $$aDoerner, Katerina
000001940 7001_ $$aGiewekemeyer, Klaus
000001940 7001_ $$aGraceffa, Rita
000001940 7001_ $$aKelsey, Oliver D.
000001940 7001_ $$aKim, Yoonhee
000001940 7001_ $$aKirkwood, Henry J.
000001940 7001_ $$aLegrand, Alexis
000001940 7001_ $$aLetrun, Romain
000001940 7001_ $$aManning, Bradley
000001940 7001_ $$aLopez Morillo, Luis
000001940 7001_ $$aMesserschmidt, Marc
000001940 7001_ $$aMills, Grant
000001940 7001_ $$aRaabe, Steffen
000001940 7001_ $$aReimers, Nadja
000001940 7001_ $$aRound, Adam
000001940 7001_ $$aSato, Tokushi
000001940 7001_ $$aSchulz, Joachim
000001940 7001_ $$aSigne Takem, Cedric
000001940 7001_ $$aSikorski, Marcin
000001940 7001_ $$aStern, Stephan
000001940 7001_ $$aThute, Prasad
000001940 7001_ $$aVagovič, Patrik
000001940 7001_ $$aWeinhausen, Britta
000001940 7001_ $$aTschentscher, Thomas
000001940 773__ $$j26$$k3$$pJ. Synchrotron Rad.$$q1-17$$tJournal of Synchrotron Radiation
000001940 790__ $$aEuXFEL staff
000001940 790__ $$aOther
000001940 85641 $$uhttp://scripts.iucr.org/cgi-bin/paper?S1600577519003308
000001940 8560_ $$fkurt.ament@xfel.eu
000001940 8564_ $$uhttps://xfel.tind.io/record/1940/files/ig5074.pdf$$s2029183
000001940 900__ $$aInstrument SPB/SFX
000001940 900__ $$aManagement Board
000001940 980__ $$aARTICLE