000001939 001__ 1939
000001939 005__ 20190730174300.0
000001939 02470 $$2DOI$$a10.1107/S1600577519002686
000001939 037__ $$aARTICLE-2019-060
000001939 041__ $$aeng
000001939 245__ $$aNative mass spectrometry provides sufficient ion flux for XFEL single-particle imaging
000001939 260__ $$bInternational Union of Crystallography$$c2019
000001939 269__ $$a2019
000001939 336__ $$aArticles
000001939 520__ $$aThe SPB/SFX instrument at the European XFEL provides unique conditions for single-particle imaging (SPI) experiments due to its high brilliance, nano-focus and unique pulse structure. Promising initial results provided by the international LCLS (Linac Coherent Light Source) SPI initiative highlight the potential of SPI. Current available injection methods generally have high sample consumption and do not provide any options for pulsing, selection or orientation of particles, which poses a problem for data evaluation. Aerosol-injector-based sample delivery is the current method of choice for SPI experiments, although, to a lesser extent, electrospray and electrospinning are used. Single particles scatter only a limited number of photons providing a single orientation for data evaluation, hence large datasets are required from particles in multiple orientations in order to reconstruct a structure. Here, a feasibility study demonstrates that nano-electrospray ionization, usually employed in biomolecular mass spectrometry, provides enough ion flux for SPI experiments. A novel instrument setup at the SPB/SFX instrument is proposed, which has the benefit of extremely low background while delivering mass over charge and conformation-selected ions for SPI.
000001939 546__ $$aEnglish
000001939 6531_ $$aSPI
000001939 6531_ $$aelectrospray Ionization
000001939 6531_ $$aX-ray free-electron lasers
000001939 6531_ $$anative MS
000001939 6531_ $$astructural biology
000001939 655__ $$aStructural biology and biocrystallography
000001939 655__ $$aX-ray scattering, X-ray optics, and instrumentation techniques
000001939 690__ $$aExperimental techniques
000001939 690__ $$aSPB/SFX experiment
000001939 690__ $$aEuXFEL facility
000001939 7001_ $$aUetrecht, Charlotte
000001939 7001_ $$aLorenzen, Kristina
000001939 7001_ $$aKitel, Matthäus
000001939 7001_ $$aHeidemann, Johannes
000001939 7001_ $$aRobinson Spencer, Jesse Huron
000001939 7001_ $$aSchlüter, Hartmut
000001939 7001_ $$aSchulz, Joachim
000001939 773__ $$tJournal of Synchrotron Radiation$$q1-7$$pJ. Synchrotron Radiat.$$k3$$j26
000001939 790__ $$aEuXFEL staff
000001939 790__ $$aOther
000001939 85641 $$uhttp://journals.iucr.org/s/issues/2019/03/00/xq5002/index.html
000001939 8560_ $$flaura.outterside@xfel.eu
000001939 8564_ $$uhttps://xfel.tind.io/record/1939/files/xq5002.pdf$$s713100
000001939 900__ $$aSample Environment
000001939 980__ $$aARTICLE