Record Details

Title:
Megahertz serial crystallography
Author(s):
Wiedorn, Max O.
Oberthür, Dominik
Bean, Richard
Schubert, Robin
Werner, Nadine
Abbey, Brian
Aepfelbacher, Martin
Adriano, Luigi
Allahgholi, Aschkan
Al-Qudami, Nasser
Andreasson, Jakob
Aplin, Steve
Awel, Salah
Ayyer, Kartik
Bajt, Saša
Barák, Imrich
Bari, Sadia
Bielecki, Johan
Botha, Sabine
Boukhelef, Djelloul
Brehm, Wolfgang
Brockhauser, Sandor
Cheviakov, Igor
Coleman, Matthew A.
Cruz-Mazo, Francisco
Danilevski, Cyril
Darmanin, Connie
Doak, R. Bruce
Domaracky, Martin
Dörner, Katerina
Du, Yang
Fangohr, Hans
Fleckenstein, Holger
Frank, Matthias
Fromme, Petra
Gañán-Calvo, Alfonso M.
Gevorkov, Yaroslav
Giewekemeyer, Klaus
Ginn, Helen Mary
Graafsma, Heinz
Graceffa, Rita
Greiffenberg, Dominic
Gumprecht, Lars
Göttlicher, Peter
Hajdu, Janos
Hauf, Steffen
Heymann, Michael
Holmes, Susannah
Horke, Daniel A.
Hunter, Mark S.
Imlau, Siegfried
Kaukher, Alexander
Kim, Yoonhee
Klyuev, Alexander
Knoška, Juraj
Kobe, Bostjan
Kuhn, Manuela
Kupitz, Christopher
Küpper, Jochen
Lahey-Rudolph, Janine Mia
Laurus, Torsten
Le Cong, Karoline
Letrun, Romain
Xavier, P. Lourdu
Maia, Luis
Maia, Filipe R. N. C.
Mariani, Valerio
Messerschmidt, Marc
Metz, Markus
Mezza, Davide
Michelat, Thomas
Mills, Grant
Monteiro, Diana C. F.
Morgan, Andrew
Mühlig, Kerstin
Munke, Anna
Münnich, Astrid
Nette, Julia
Nugent, Keith A.
Nuguid, Theresa
Orville, Allen M.
Pandey, Suraj
Pena, Gisel
Villanueva-Perez, Pablo
Poehlsen, Jennifer
Previtali, Gianpietro
Redecke, Lars
Riekehr, Winnie Maria
Rohde, Holger
Round, Adam
Safenreiter, Tatiana
Sarrou, Iosifina
Sato, Tokushi
Schmidt, Marius
Schmitt, Bernd
Schönherr, Robert
Schulz, Joachim
Sellberg, Jonas A.
Seibert, M. Marvin
Seuring, Carolin
Shelby, Megan L.
Shoeman, Robert L.
Sikorski, Marcin
Silenzi, Alessandro
Stan, Claudiu A.
Shi, Xintian
Stern, Stephan
Sztuk-Dambietz, Jola
Szuba, Janusz
Tolstikova, Aleksandra
Trebbin, Martin
Trunk, Ulrich
Vagovic, Patrik
Ve, Thomas
Weinhausen, Britta
White, Thomas A.
Wrona, Krzysztof
Xu, Chen
Yefanov, Oleksandr
Zatsepin, Nadia
Zhang, Jiaguo
Perbandt, Markus
Mancuso, Adrian P.
Betzel, Christian
Chapman, Henry
Barty, Anton
Affiliation(s):
EuXFEL staff, EuXFEL user
Author group:
Control and Analysis Software
Detector Development
IT and Data Management
Sample Environment
Instrument SPB/SFX
Topic:
Scientific area:
Abstract:
The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.
Imprint:
2018
Journal Information:
Nature Communications, 9 (1), 4025 (2018)
Related external records:
Language(s):
English


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 Record created 2018-10-03, last modified 2019-02-06

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