Record Details

Title:
Attachment of Norovirus to Histo Blood Group Antigens: A Cooperative Multistep Process
Abstract:
Human noroviruses recognize histo blood group antigens (HBGAs) as cellular attachment factors. Recently, it has been discovered that norovirus infection can be significantly enhanced by HBGA binding. Yet the attachment process and how it promotes host-cell entry is only poorly understood. The binding of a norovirus protruding (P) domain of a predominant GII.4 Saga strain to HBGAs at atomic resolution was studied. So far, independent and equivalent multiple binding sites were held responsible for attachment. Using NMR experiments we show that norovirus-HBGA binding is a cooperative multi-step process, and native mass spectrometry reveals four instead of two HBGA binding sites per P-dimer. An accompanying crystallographic study has disclosed four instead of two l-fucose binding sites per P-dimer of a related GII.10 strain1 further supporting our findings. We have uncovered a novel paradigm for norovirus-HBGA recognition that will inspire further studies into norovirus–host interactions.
Imprint:
Weinheim, Wiley-VCH, 2015
Journal Information:
Angew. Chem. Int. Ed., 54, 41, 12014–12019 (2015)
ISSN:
0044-8249
0570-0833
1433-7851
1521-3773
Related external records:
DOI: 10.1002/anie.201505672
WOS: WOS:000363396000020
Language(s):
English
Record appears in:


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 Record created 2016-10-11, last modified 2018-12-19

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