In vivo protein crystallization opens new routes in structural biology
Koopmann, R.; Cupelli, K.; Redecke, L.; Nass, K.; DePonte, D. P.; White, T. A.; Stellato, F.; Rehders, D.; Liang, M.; Andreasson, J.; Aquila, A.; Bajt, S.; Barthelmess, M.; Barty, A.; Bogan, M. J.; Bostedt, C.; Boutet, S.; Bozek, J. D.; Caleman, C.; Coppola, N.; Davidsson, J.; Doak, R. B.; Ekeberg, T.; Epp, S. W.; Erk, B.; Fleckenstein, H.; Foucar, L.; Graafsma, H.; Gumprecht, L.; Hajdu, J.; Hampton, C. Y.; Hart- mann, A.; Hartmann, R.; Hauser, G.; Hirsemann, H.; Holl, P.; Hunter, M. S.; Kassemeyer, S.; Kirian, R. A.; Lomb, L.; Maia, F. R. N. C.; Kimmel, N.; Martin, A. V.; Messerschmidt, M.; Reich, C.; Rolles, D.; Rudek, B.; Rudenko, A.; Schlichting, I.; Schulz, Joachim; Seibert, M. M.; Shoeman, R. L.; Sierra, R. G.; Soltau, H.; Stern, S.; Struder, L.; Timneanu, N.; Ullrich, J.; Wang, X.; Weidenspointner, G.; Weierstall, U.; Williams, G. J.; Wunderer, C. B.; Fromme, P.; Spence, J. C. H.; Stehle, T.; Chapman, H. N.; Betzel, C.; Duszenko, M.
2012
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Abstract
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
Details
Title
In vivo protein crystallization opens new routes in structural biology
Author
Koopmann, R.
Cupelli, K.
Redecke, L.
Nass, K.
DePonte, D. P.
White, T. A.
Stellato, F.
Rehders, D.
Liang, M.
Andreasson, J.
Aquila, A.
Bajt, S.
Barthelmess, M.
Barty, A.
Bogan, M. J.
Bostedt, C.
Boutet, S.
Bozek, J. D.
Caleman, C.
Coppola, N.
Davidsson, J.
Doak, R. B.
Ekeberg, T.
Epp, S. W.
Erk, B.
Fleckenstein, H.
Foucar, L.
Graafsma, H.
Gumprecht, L.
Hajdu, J.
Hampton, C. Y.
Hart- mann, A.
Hartmann, R.
Hauser, G.
Hirsemann, H.
Holl, P.
Hunter, M. S.
Kassemeyer, S.
Kirian, R. A.
Lomb, L.
Maia, F. R. N. C.
Kimmel, N.
Martin, A. V.
Messerschmidt, M.
Reich, C.
Rolles, D.
Rudek, B.
Rudenko, A.
Schlichting, I.
Schulz, Joachim
Seibert, M. M.
Shoeman, R. L.
Sierra, R. G.
Soltau, H.
Stern, S.
Struder, L.
Timneanu, N.
Ullrich, J.
Wang, X.
Weidenspointner, G.
Weierstall, U.
Williams, G. J.
Wunderer, C. B.
Fromme, P.
Spence, J. C. H.
Stehle, T.
Chapman, H. N.
Betzel, C.
Duszenko, M.
Cupelli, K.
Redecke, L.
Nass, K.
DePonte, D. P.
White, T. A.
Stellato, F.
Rehders, D.
Liang, M.
Andreasson, J.
Aquila, A.
Bajt, S.
Barthelmess, M.
Barty, A.
Bogan, M. J.
Bostedt, C.
Boutet, S.
Bozek, J. D.
Caleman, C.
Coppola, N.
Davidsson, J.
Doak, R. B.
Ekeberg, T.
Epp, S. W.
Erk, B.
Fleckenstein, H.
Foucar, L.
Graafsma, H.
Gumprecht, L.
Hajdu, J.
Hampton, C. Y.
Hart- mann, A.
Hartmann, R.
Hauser, G.
Hirsemann, H.
Holl, P.
Hunter, M. S.
Kassemeyer, S.
Kirian, R. A.
Lomb, L.
Maia, F. R. N. C.
Kimmel, N.
Martin, A. V.
Messerschmidt, M.
Reich, C.
Rolles, D.
Rudek, B.
Rudenko, A.
Schlichting, I.
Schulz, Joachim
Seibert, M. M.
Shoeman, R. L.
Sierra, R. G.
Soltau, H.
Stern, S.
Struder, L.
Timneanu, N.
Ullrich, J.
Wang, X.
Weidenspointner, G.
Weierstall, U.
Williams, G. J.
Wunderer, C. B.
Fromme, P.
Spence, J. C. H.
Stehle, T.
Chapman, H. N.
Betzel, C.
Duszenko, M.
Affiliation
EuXFEL guest, EuXFEL staff
Author Group
Optical Lasers
Advanced Electronics
Instrument SPB/SFX
Sample Environment
Detector Development
Advanced Electronics
Instrument SPB/SFX
Sample Environment
Detector Development
Keywords
Imprint
London [u.a.] Nature Publishing Group, 2012, Nature Publishing Group
Date
2012
Publication Information
Nat. Methods, 9, 259-262 (2012)
ISSN
1548-7105
1548-7091
1548-7091
Related external records
View record in PubMed
pmc: pmc:PMC3429599
WOS: WOS:000300890400020
DOI: https://doi.org/10.1038/nmeth.1859
pmc: pmc:PMC3429599
WOS: WOS:000300890400020
DOI: https://doi.org/10.1038/nmeth.1859
Related proposal number
POF II
Content Type
Articles
Language
English
Funding Information
Experiments at CFEL (POF2-544)
Record Appears in
Groups > Detectors and data acquisition > Electronic & Electrical Engineering
Groups > Scientific instruments and equipment > Sample Environment
Groups > Scientific instruments and equipment > Optical Lasers
Groups > Detectors and data acquisition > Detector Development
Types > Articles
Years > 2012
Advanced Electronics (AE)
Groups > Scientific instruments and equipment > Sample Environment
Groups > Scientific instruments and equipment > Optical Lasers
Groups > Detectors and data acquisition > Detector Development
Types > Articles
Years > 2012
Advanced Electronics (AE)